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spam1 antibody  (Novus Biologicals)


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    Novus Biologicals spam1 antibody
    Spam1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spam1 antibody/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    spam1 antibody - by Bioz Stars, 2026-03
    94/100 stars

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    Image Search Results


    ( A and B ) Size and zeta potential of anti-SPAM1 NPs and anti-SPAM1-mel-NPs immediately following preparation. ( C ) Fluorescence of sperm-bound blank NPs and anti-SPAM1 NPs following incubation with sperm for 30 minutes and removal of unbound NPs by density gradient centrifugation. Control samples did not contain sperm and were used to determine background fluorescence due to remaining unbound NPs. ( D ) Standard curve of blank NP and anti-SPAM1 NP fluorescence used for quantification of sperm binding. Error bars represent S.D. of n = 3 replicates, NS p>0.05, *** p<0.001. ( E , F , G and H ) Brightfield and fluorescence images of semen samples following addition of 3×10 10 blank NPs or anti-SPAM1 NPs. Scale bar = 50 µm.

    Journal: PLoS ONE

    Article Title: Nanoparticle Incorporation of Melittin Reduces Sperm and Vaginal Epithelium Cytotoxicity

    doi: 10.1371/journal.pone.0095411

    Figure Lengend Snippet: ( A and B ) Size and zeta potential of anti-SPAM1 NPs and anti-SPAM1-mel-NPs immediately following preparation. ( C ) Fluorescence of sperm-bound blank NPs and anti-SPAM1 NPs following incubation with sperm for 30 minutes and removal of unbound NPs by density gradient centrifugation. Control samples did not contain sperm and were used to determine background fluorescence due to remaining unbound NPs. ( D ) Standard curve of blank NP and anti-SPAM1 NP fluorescence used for quantification of sperm binding. Error bars represent S.D. of n = 3 replicates, NS p>0.05, *** p<0.001. ( E , F , G and H ) Brightfield and fluorescence images of semen samples following addition of 3×10 10 blank NPs or anti-SPAM1 NPs. Scale bar = 50 µm.

    Article Snippet: To biotinylate the monoclonal anti-SPAM1 antibody, 0.2 mg/ml antibody was mixed with 8.24 µL 1 mM Biotin-NHS (Thermo Scientific, Rockford, IL) and incubated at room temperature for 30 minutes.

    Techniques: Fluorescence, Incubation, Gradient Centrifugation, Binding Assay

    ( A and B ) Size and zeta potential of anti-SPAM1 NPs and anti-SPAM1-mel-NPs immediately following preparation. ( C ) Fluorescence of sperm-bound blank NPs and anti-SPAM1 NPs following incubation with sperm for 30 minutes and removal of unbound NPs by density gradient centrifugation. Control samples did not contain sperm and were used to determine background fluorescence due to remaining unbound NPs. ( D ) Standard curve of blank NP and anti-SPAM1 NP fluorescence used for quantification of sperm binding. Error bars represent S.D. of n = 3 replicates, NS p>0.05, *** p<0.001. ( E , F , G and H ) Brightfield and fluorescence images of semen samples following addition of 3×10 10 blank NPs or anti-SPAM1 NPs. Scale bar = 50 µm.

    Journal: PLoS ONE

    Article Title: Nanoparticle Incorporation of Melittin Reduces Sperm and Vaginal Epithelium Cytotoxicity

    doi: 10.1371/journal.pone.0095411

    Figure Lengend Snippet: ( A and B ) Size and zeta potential of anti-SPAM1 NPs and anti-SPAM1-mel-NPs immediately following preparation. ( C ) Fluorescence of sperm-bound blank NPs and anti-SPAM1 NPs following incubation with sperm for 30 minutes and removal of unbound NPs by density gradient centrifugation. Control samples did not contain sperm and were used to determine background fluorescence due to remaining unbound NPs. ( D ) Standard curve of blank NP and anti-SPAM1 NP fluorescence used for quantification of sperm binding. Error bars represent S.D. of n = 3 replicates, NS p>0.05, *** p<0.001. ( E , F , G and H ) Brightfield and fluorescence images of semen samples following addition of 3×10 10 blank NPs or anti-SPAM1 NPs. Scale bar = 50 µm.

    Article Snippet: To target sperm, anti-sperm adhesion molecule 1 (anti-SPAM1) antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) were biotinylated and complexed with avidin NPs.

    Techniques: Zeta Potential Analyzer, Fluorescence, Incubation, Gradient Centrifugation, Control, Binding Assay